Technical Data
N2028-50R
Nephrin, phosphorylated (Tyr1217) (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)
Description:
Nephrin (Renal glomenelus specific adhesion receptor) is a kidney glomerular filtration barrier protein that is an essential component of the interpodocyte-spanning slit diaphragm. Nephrin is located only in visceral glomerular epithelial cells, where it is targeted to intercellular junction of
mature podocyte foot processes (1). Mutations in the nephrin gene are associated with congenial nephritic syndrome (NPHS1) (2).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25,000-1:50,000
Immunoprecipitation: 1:20000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H483Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues surrounding tyrosine 1217 of mouse Nephrin.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes mouse Nephrin when phosphorylated at Tyr1217. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holzman LB, et al. Kidney Int 56(4) :1481-91, 1999 2. Jones, et al. Nature 440(7085) :818-23, 2006
tyrosine kinase (vSrc) (C) HEK293T cells transfected