Technical Data
N2028-50T
Nephrin, CT (Renal Glomerulus-specific Cell Adhesion Receptor, NPHS1, NPHN)
Description:
Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomer uli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit- diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa from near the C-terminus of human Nephrin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes human Nephrin. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kestila M, et al (1998) Mol. Cell. 1:575-582. 2. Tryggvason K (1999) J. Am. Soc. Nephrol. 10:2440-5. 3. Tryggvason K, et al (2001) Curr, Opin. Nephrol. Hypertens. 10:543-9.