Technical Data
N2028-62
Nephrin Related Protein 1 (NEPH1)
Description:
Nephrin is encoded by NPHS1 gene (chromosome 19q13.1), the core protein of the interpodocytes slit diaphragm of kidney glomerulus, these podocytes are highly differentiated with characteristic interdigitating foot processes covering the outer glomerular basement membrane, the space between these foot processes is spanned by a tight junction to provide the large surface area for filtration. The mutated NPHS1 gene in congenital nephrotic syndrome of Finnish type, a human disease that leads to massive proteinuria in utero and nephrosis at birth. Nephrin is a transmembrane protein of immunoglobulin super family with 8 Ig like domains, identified in human (1241aa), rat (1252aa), and mouse (1256aa).

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See N2028-62A.
Western Blot (ECL): 1-10ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
N2028-62A: Nephrin Related Protein 1, Rat, Control Peptide (NEPH1)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic 16aa peptide from rat NEPH1. Epitope: ~C-terminus, Cytoplasmic domain.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.
Specificity:
Recognizes rat NEPH1. Species Sequence Homology: human, mouse - 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lorenz Sellin et al (2002) FASEB journal article 10.1096, published online; 2. Heli Putaala et al (2001) Human Mol. Gen.10, 1-8; 3. Dorbit B.Donoviel et al (2001) Mol and Celluar Biol 21, 4829-4836; 4. David B. Kershaw et al (1997) JBC 272, 15708-15714. 5. Wang L, 2005, J Am Soc Nephrol 16: 36113622, 2005; 6. WB Garovic VD, 2007, Nephrol Dial Trans.(2007) 22: 1136; 7. IHC Neto JS, 2005, Am J Physiol Renal Physiol, 290: F324 - F334,; 8. F Yuan H, 2002, J Am. Soc. Nephrol. 13, 946-956, WB, IHC 9. Leissring MA, 2003, Neuron, 40, 1087-1093.