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Technical Data |
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N2050-05A |
Nerve Growth Factor, 2.5S, Murine (NGF, Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Nerve growth factor (NGF) is a protein secreted by the target of a neuron that stimulates the growth and differentiation of the sympathetic and certain sensory nerves. It consists of 3 subunits, alpha, beta and gamma, with the beta subunit having sole responsible for nerve and growth stimulating activity. Source: Nerve Growth Factor 2.5S, from mouse submaxillaries. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Mouse submaxillaries Purity: ~95% (SDS PAGE). Form: Supplied as a lyophilized powder in tris buffered saline. Reconstitute with sterile ddH2O. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Boccini, V. & Angeletti, P. U. (1969) The nerve growth factor: purification as a 30,000-molecular-weight protein. Proc. Natl. Acad. Sci. US.A. 64: 787-794. 2. Green, L. A. (1977) A quantitative bioassay for nerve growth factor (NGF) activity employing a clonal pheochromocytoma cell line. Brain Res. 133: 350-353.
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