Technical Data
Nerve Growth Factor, 2.5S, Murine (NGF, Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb)
Molecular Biology Storage: -20CShipping: Blue Ice
Nerve growth factor (NGF) is a protein secreted by the target of a neuron that stimulates the growth and differentiation of the sympathetic and certain sensory nerves. It consists of 3 subunits, alpha, beta and gamma, with the beta subunit having sole responsible for nerve and growth stimulating activity.

Nerve Growth Factor 2.5S, from mouse submaxillaries.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Mouse submaxillaries
Purity: ~95% (SDS PAGE).
Form: Supplied as a lyophilized powder in tris buffered saline. Reconstitute with sterile ddH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Boccini, V. & Angeletti, P. U. (1969) The nerve growth factor: purification as a 30,000-molecular-weight protein. Proc. Natl. Acad. Sci. US.A. 64: 787-794. 2. Green, L. A. (1977) A quantitative bioassay for nerve growth factor (NGF) activity employing a clonal pheochromocytoma cell line. Brain Res. 133: 350-353.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.