Technical Data
N2050-06
Nerve Growth Factor, 7S (NGF)
25ug
4x25ug
Molecular Biology Storage: -20CShipping: Blue Ice
Dose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells: 10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development.3 7S NGF is also needed for sympathetic nerve cells development and maintenance.

Biological Activity:
Dose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells: 10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development. 7S NGF is also needed for sympathetic nerve cells development and maintenance.

Extinction Coefficient
( E1%, 280nm, pH 7.0): 14.2

Sterility:
Sterilized through a 0.2um membrane filter and packaged aseptically.

NGF Assay:
1. Coat 6 well dishes with rat tail collagen type I, at a concentration of 2.5-5.0ug/cm2. Incubate for one hour at 37C. Aspirate and rinse 2 times with PBS before adding cell suspension.
2. Prepare a cell suspension to yield 2x10e4 cells/ml in RPMI-1640/10% horse serum/ 5% FBS. Add 2mls of cell suspension to each well.
3. Prepare NGF as described above under Rehydration to a final concentration of 1ug/ml. Add appropriate amounts of NGF to test at 0, 5, 10 and 20ng/ml final concentration.
4. Incubate plates at 37C for 3-5 days.
5. Record neurite outgrowth at each NGF concentration.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
130-140kD
Source: Male mouse submaxillary glands
Purity: Purified by gel filtration. Does not contain detectable amounts of EGF
Concentration: ~1mg/ml
Form: Supplied as a liquid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bocchini, V. and P.U. Angeletti, Proc. Natl. Acad. Sci. 64: 787-794, 1969. 2. Bocchini, V., Eur. J. Biochem. 15: 127-131, 1970. 3. Levi-Montalcini, R. and P.U. Angeletti, Physiol. Rev. 48: 534-569, 1968. 4. Marx, J.L., Science 232: 1341-1342, 1986.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.