		Technical Data
N2050-06F	Nerve Growth Factor, beta (NGF, Beta Nerve Growth Factor, Beta Nerve Growth Factor Precursor, Beta NGF, HSAN5, Nerve Growth Factor beta, Nerve Growth Factor beta Polypeptide, Nerve Growth Factor beta Subunit, NGFB)

Description:
Nerve Growth Factor is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested.

Recommended Dilution:
ELISA: 1-3ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 1-3ug/ml
Inhibition of Biological Activity In vitro: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE).

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder. Reconstitute in 500ul sterile ddH2O.

Specificity:

Recognizes mouse beta Nreve Growth Factor. A crossreactivity of <1% has been shown to recombinant human BDNF, NT3, NT4/5 by ELISA. Completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Species Crossreactivity: Rat, human and avian. Does not crossreact with bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240. ; 2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102. ; 3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75.