Technical Data
N2050-06P
Nerve Growth Factor, beta (CT) (NGF, Beta Nerve Growth Factor, Beta Nerve Growth Factor Precursor, Beta NGF, HSAN5, Nerve Growth Factor beta, Nerve Growth Factor beta Polypeptide, Nerve Growth Factor beta Subunit, NGFB)
Description:
Nerve growth factor-beta (NGF-b) is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, formed by two 13.5kD polypeptide monomers (238 total amino acids). The three disulfide bonds are required for biological activity.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500. Fetal thyroid cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2050-06P. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry (Paraffin): 1:50-1:100. Detected NGF-beta in human brain tissue
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Positive Control:
Fetal thyroid cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9i179Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues near the C-terminus of human NGF-beta. Cellular Localization: Secreted.
Purity:
Supernatant
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human NGF-beta at ~32kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Levi-Montalcini, R. (1997) Nature 237:1154-1162. 2. Ullrich, A., et al. (1983) Nature 303:821-825.