		Technical Data
N2050-10	Nerve Growth Factor, beta, Recombinant, Human (NGFb)	5ug 20ug

Growth Factors, Cytokines

Storage: -20°CShipping: RT

Nerve growth factor-b (b-NGF) is structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cystein-knot family of growth factors that assume stable dimeric structures. b-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. b-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival.

The functional form of human b-NGF is a noncovalently disulfide-linked homodimer, of two 13.5kD polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.

AA Sequence:
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR

Biological Activity:
The ED50 as determined by the ability of NGFb to stimulate chick E9 DRG neurite outgrowth is 1.0ng/ml, corresponding to a specific activity of 1x10e6 units/mg.

Responding Cells (partial list):
Nerve, glial cells

Concentration Range for Most in vitro Applications:
0.5-100ng/ml

Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1%BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
27.0kD

Source: E. coli
Purity: ~ 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized protein without additives. Reconstitution: Reconstitute in sterile dH2O, 0.1% BSA to 0.1-1mg/ml. Do not vortex.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.