		Technical Data
N2050-10B	Nerve Growth Factor, beta Recombinant, Human (NGFb)	20ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Recombinant human nerve growth factor beta is a homodimer, glycosylated, polypeptide chain containing 2 identical 119aa and having a molecular mass of 16.9kD. The sequence of the first five N-terminal amino acids was found to be Ser-Ser-Ser-His-Pro.

Source:
Human Nerve Growth Factor beta, from CHO cells.

Molecular Weight:
~16.9kD

Biological Activity:
The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be <1ng/ml, corresponding to a specific activity of >1x10e6Units/mg.

Endotoxin: 0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
16.9kD

Source: CHO Cells
Purity: 98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Form: Supplied as a lyophilized powder from 30mM sodium acetate, pH 5.5, 1% HSA. Reconstitute with sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.