Technical Data
N2050-10C
Nerve Growth Factor, beta Recombinant, Human (NGFb)
5ug
20ug
100ug
Molecular Biology Storage: -70CShipping: Blue Ice
Human NGF-beta is a neurotrophic factor related to BDNF, NT-3 and NT-4. Human NGF-beta acts as a neurotrophic factor through its receptor beta-NGFR, and is involved in the development and sustaining the sensory and sympathetic nervous systems. H-NGFbeta also is also involved in the growth and differentiation of B lymphocytes and B-cell survival. Human NGF-beta is a noncovalently disulfide-linked homodimer, consisting of 121 amino acids with a molecular weight of two 13.6kD polypeptide monomers.

Endotoxin: <0.01ng/ug or <0.1EU/ug as per LAL

AA Sequence:
MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRR

Biological Activity: Biological acitivity is determined by the ability to stimulate chick E9 DRG neurite growth. The ED50 is < 1.0ng/ml, corresponding to a specific activity of > 1x10e6 units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
13.6 kD
Source: Recombinant, human, E. coli
Purity: 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.