Technical Data
N2050-11T
Nerve Growth Factor Receptor, p75 (NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16)
Description:
p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins, but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. p75NTR is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5ug/ml
Immunohistochemistry (Frozen sections): 1-5ug/ml; 4% paraformaldehyde fixed.
Immunoflorescence: 20ug/ml
Flow Cytometry: 20ug/ml
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G395Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Extracellular domain of human p75NTR
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human Nerve Growth Factor Receptor, p75. Species Crossreactivity: mouse, guinea pig and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rogers, M.L., et al., J. Neurosci. Methods. 158(1): 109-120 (2006). 2. Matusica, D., et al., J. Neurosci. Res. 86(3): 553-565 (2008). 3. Huh, C.Y., et al., J. Neurosci. 28(6): 1404-1409 (2008). 4. Lagares, A., et al., J. Neurosci. 27(30): 7939-7953 (2007).