Technical Data
N2060-04
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Description:
Nestin (200 kD) is an intermediate filament protein found in developing rat brain. Functionally similar to other IF proteins but very different in aa sequence.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000-1:70,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide, ALETESQDSAEPSGSEE, corresponding human nestin
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.