Technical Data
N2060-04B
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Description:
Nestin is an intermediate filament involved in cytoskeletal organisation. Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.

Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:100-1:200
Immunohistochemistry (paraffin): 1:100-1:200
Immunoblot: 1:500-1:5,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Human Nestin.
Purity:
Serum
Form
Supplied as a liquid. Preservative free.
Specificity:
Recognizes human Nestin. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Messam, C.A. et al (2000) Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp Neurol. 161: 585-596. 2. Vogel, W. (2003) Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 88: 126-133. 3. Humphrey, R.K. et al. (2003) Characterization and isolation of promoter-defined nestin-positive cells from the human fetal pancreas. Diabetes. 52: 2519-2525.