		Technical Data
N2060-04E	Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Description:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10,000-1:30,000
Western Blot: 1:20,000-1:50,000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Positive Control:
Mouse brain

Marker:
Oligodendrocyte Marker

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgY		Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Mouse	Chicken

Concentration:

~0.25mg/ml

Immunogen:

Three different affinity-purified anti-peptide antibodies were combined to make this product. Cellular localization: cytoplasmic.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM PBS pH 7.2, 0.02% sodium azide.

Specificity:

Recognizes mouse nestin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Alam G, Cui H, Shi H, et al. MYCN Promotes the Expansion of Phox2B-Positive Neuronal Progenitors to Drive Neuroblastoma Development. Am J Pathol 2009;175(2):856-866. [pubmed: 19608868]