Technical Data
N2060-04E
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Description:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10,000-1:30,000
Western Blot: 1:20,000-1:50,000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Positive Control:
Mouse brain

Marker:
Oligodendrocyte Marker

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
250ul4C Do Not FreezeBlue IceMouseChicken
Concentration:
~0.25mg/ml
Immunogen:
Three different affinity-purified anti-peptide antibodies were combined to make this product. Cellular localization: cytoplasmic.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes mouse nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alam G, Cui H, Shi H, et al. MYCN Promotes the Expansion of Phox2B-Positive Neuronal Progenitors to Drive Neuroblastoma Development. Am J Pathol 2009;175(2):856-866. [pubmed: 19608868]