		Technical Data
N2060-04H	Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Description:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Westerm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Immnohistochemistry: Paraffin and Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	9A19	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~2mg/ml

Immunogen:

A fragment of the human nestin protein corresponding to residues 1464-1614.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid.

Specificity:

Recognizes human nestin. It does not crossreact with rodent protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Messam, C.A., et al. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp. Neurology. 161: 585-596, 2000.