Technical Data
N2060-04L
NES (S1409) (Nestin, Nbla00170)
Description:
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to aa1395-1425 surrounding S1409 of human NES, conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human NES.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yaworsky, P.J., et al., Dev. Biol. 205(2):309-321 (1999). 2. Dahlstrand, J., et al., J. Cell. Sci. 103(Pt 2), 589-597 (1992).