Technical Data
N2060-05C
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Description:
Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F211Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human Nestin.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Nestin. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hockfield,S. and McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 5: 3310-3328.