Technical Data
N2060-05E
NES (Nestin, Nbla00170)
Description:
Nestin (NES) is an intermediate filament protein. Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12C1.3A11Affinity Purified
SizeStorageShippingSourceHost
50ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
A 150aa fragment from the cloned human Nestin conjugated to GST.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes human Nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.