Technical Data
N2060-06
Nestin (NES, FLJ21841, Nbla00170)
Description:
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.25ug/ml
Immunohistochemistry: 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the N-terminal region of human Nestin, conjugated to KLH.
Purity:
Purified by Protein G afinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Nestin at ~177kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yaworsky, P.J., et al., Dev. Biol. 205(2):309-321 (1999). 2. Dahlstrand, J., et al., J. Cell. Sci. 103 (Pt 2), 589-597 (1992).