Technical Data
N2060-08
Nestin
Description:
Mouse Nestin is an approximately 207kD protein (1864aa) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde-fixed tissues.
Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde-fixed cells.
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
300ug-20°CBlue IceMouseChicken
Concentration:
~0.3mg/ml
Immunogen:
Three synthetic peptides corresponding to different regions of the mouse Nestin (NP_057910) (KLH). Species Sequence Homology: rat (NM_012987, NCBI).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes mouse Nestin. Note that the sequences of the mouse and human Nestin genes are divergent, and it is unlikely that antibodies raised against the mouse sequence would recognize human Nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.