Technical Data
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes) (Biotin)
Nestin is a 240kD class VI intermediate filament protein that was first identified in neuroepithelial stem cells. It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% aa sequence homology with the mouse protein.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20CBlue IceRatGoat
Not determined
Purified, E. coli-derived, recombinant rat Nestin (rrNestin; aa544-776).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Recognizes rat Nestin. Species sequence homology: mouse (76%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310-3328. 2. Lendahl, U. et al. (1990) Cell 60:585-595.