Technical Data
N2171-60
Neurokinin B Receptor (NK-3 Receptor)
Description:
The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the C-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:10-1:2000
Immunohistochemistry (Frozen): 1:10-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
150ul-20°CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the C-terminus of rat Neurokinin B Receptor (NK-3 Receptor) conjugated to bovine thyroglobulin.
Purity:
Serum
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes the rat NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as preabsorption studies with NK-3 peptide. Species Crossreactivity: mouse and guinea pig in brain, spinal cord and ileum. Does not react with human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Burke MC, Letts PA, Krajewski SJ, et al. J Comp Neurol. 2006 Oct 10;498(5):712-26. 2. Tooney, Paul A, et al. Neurosci Lett. 2000 Apr 14;283(3):185-8. 3. Yip, J, et al. Regul Pept. 2001 Apr 2;98(1-2):55-62. 4. Dick, O. Neuron. 2003 Mar 6;37(5):775-86. 5. Howe, H, et al. J Neurosci. 2004 Nov 10;24(45):10103-10. 6. Feigenspan, A, et al. J Neurosci. 2004 Mar 31;24(13):3325-34. 7. Haley, GE, Flynn, FW. Am J Physiol Regul Integr Comp Physiol. 2006 May;290(5):R1242-50. Epub 2005 Dec 15. 8. Oh EC, Khan N, Novelli E, et al. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1679-84.