Technical Data
N2175-01M
Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase, Enolase 2, ENO2)
Description:
Enolase is a 48kD, glycolytic enzyme that catalyzes the reaction pathway between 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The a-subunit is expressed in most tissues and the b-subunit only in muscle. The g-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:300 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas, IMR cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminus of human NSE.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human NSE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.