Technical Data
N2175-02N
Neuron Specific Enolase (NSE, 2-phospho-D-glycerate Hydrolyase, Enolase 2, Enolase-2, Eno2, ENOG, Enolase 2 (gamma, neuronal), Gamma Enolase, Neural Enolase, Neuron Specific gamma Enolase)
Description:
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Cellular Localization: Cytoplasmic

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9A59Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified Neuron Specific Enolase from human brain.
Purity:
Purified by Protein G affinity chromatography.
Form
PBS, pH 7.4 containing 0.1% sodium azide
Specificity:
Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.