Technical Data
N2175-02Z
Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase, Enolase 2, ENO2)
Description:
Enolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway. The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma.The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin.

Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
Mab10K292Ascites
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
His-tagged recombinant human gamma enolase purified from E.coli.
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human gamma Enolase. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.