Technical Data
N2175-05
Neuron Specific Enolase (NSE)
Description:
Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD), and gamma (46kD). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2ug/mg protein lysate. Native and denatured. Use Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/Parafin): 1:800-1:1600 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
IMR5 cells, pancreas.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.468Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified human Neuron Specific Enolase. Cellular Localization: Cytoplasmic. MW of Antigen: 46kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human neuron-specific enolase (NSE). Shows no crossreactivity with the alpha or beta subunits of NSE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blumenfeld, W., et al., Archives of Pathology and Laboratory Medicine 120(5): 478-481 (1996). 2. Fantini, F., Johansson, O., Experimental Dermatology 4(6): 365-371 (1995). 3. Fukunaga, M., Shinozaki, N., Pathology International 45(7): 513-519 (1995).