Technical Data
N2176-63G
Neuropeptide Y Y2 Receptor (NPY Y2R)
Description:
Several subtypes of NPY Receptors have been described of which the cloned Y1-, Y2- and Y5-receptors, members of the superfamily of G-protein coupled receptors, appear to be the endogenous binding sites for NPY. The Y(2) receptor is the predominant Neuropeptide Y (NPY) receptor subtype in the brain. Y(2) receptor-mediated effects have been reported principally based on the observations using the C-terminal fragments of NPY. Recent studies indicate an involvement of the receptor in food intake, gastrointestinal motility, cardiovascular regulation, and neuronal excitability.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:500-1:1000; Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS. Tissues were dissected and stored overnight in 10% sucrose, PBS.
Immunofluorescence (Indirect): Incubate slides with blocking buffer for 1 hour at RT. Dilute N2176-63G with blocking buffer. Remove blocking buffer from slide and incubate with N2176-63G for 18-24 hours at 4C. Rinse slides 3X and then incubate with appropriate secondary antibody for 1 hour at RT. Rinse slides again 3X and set coverslip. Examine staining using fluorescence microscopy.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide sequence corresponding to the C-terminus of NPY Y2R (KLH-C-TDSFSEATNV-COOH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes mouse Neuropeptide Y Y2 Receptor (NPY Y2R). Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The Journal of Neuroscience 28(45): 11,583-11,592 (2008).