Technical Data
N2254-01G
Neutrophils (Allotypic Marker) (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Y3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10F229Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Cultured bone marrow cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes a polymorphic 40kD antigen expressed by murine polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hirsch, S. and Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by a monoclonal antibody 7/4. Immunogenetics 18: 229-239.2. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Opin. Microbiol. Immunol. 181: 1-37.