Technical Data
N2254-01G
Neutrophils (Allotypic Marker) (FITC)
Description:
Ly-6B.2 is a ~25-30kD GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kD (Rosas et al.2010).

N2254-01G has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:10. 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Y3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10F229Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Cultured bone marrow cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the Ly-6B.2 antigen. Crossreactivity: 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO). Does not react with A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hirsch, S. and Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by a monoclonal antibody 7/4. Immunogenetics 18: 229-239.2. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Opin. Microbiol. Immunol. 181: 1-37. 3. Rosas, M. et al. (2010) The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J. Leukoc. Biol. 88: 169-180. 4. Word, R.A. et al. (2005) Transgene insertion on mouse chromosome 6 impairs function of the uterine cervix and causes failure of parturition. Biology of Reproduction. 73: 1046-1056. 5. Locke, L.W. (2009) A novel neutrophil-specific PET imaging agent: cFLFLFK-PEG-64Cu. J Nucl Med. 50: 790-7. 6. Allam, R. et al. (2011) Cyclic Polypeptide and Aminoglycoside Antibiotics Trigger IL-1{beta} Secretion by Activating the NLRP3 Inflammasome. J Immunol. 186: 2714-8.