Technical Data
N2254-01N
Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (PE)
Description:
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre- monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

Applications:
Suitable for use in Flow Cytometry, FLISA, Functional Studies and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Immunohistochemistry (Paraffin): Blocking with 20% normal rabbit serum
Flow Cytometry: 10ul/250.000 cells in a 100ul total staining volume
Functional Studies: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection
Optimal dilutions to be determined by the researcher.

Positive Control: Mouse neutrophils

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b13A63Affinity Purified
SizeStorageShippingSourceHost
25Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
As reported
Immunogen:
Purified BALB/c mouse neutrophils
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes mouse Ly-6G and Ly-6C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lopez, A et al; Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies, Brit J Haematology 1984, 57: 489 2. McLaren, D et al; Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol 1987, 9: 667. 3. Van Lent, P et al; Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice. J Leukoc Biol 1997, 61: 267. 4. Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546. 5. Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628. 6. Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883. 7. Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195. 8. Navarini, A et al; Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 2009, 106: 7107.