Technical Data
N2350-09J
Nerve Growth Factor, pro (Pro-brain nerve growth factor, proNGF, NGF)
Description:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.

Applications:
Suitable for use in Immunohistochemistry, Immnunofluorescence, ELISA, Western Blot and Neutralization. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanSheep
Concentration:
Not determined
Immunogen:
The recombinant prodomain fragment of human nerve growth factor.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human proNGF. Does not crossreact with proBDNF, proNT-3 or mature NGF. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.