Technical Data
N2375-21A
NFAT2 (NFATC1, Nuclear Factor of Activated T-cells)
Description:
The Nuclear Factor of Activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively coordinate the immune response. Two forms of NFAT, the cytosolic (NFATc1) and the pre-existing NFATc2 (formerly NFATp), have been recognized on separate genes, and show sequence similarity with the Dorsal/Rel family of transcription factors. Studies indicate that NFATc is required for interleukin (IL-2) gene expression, is found primarily in lymphoid tissues and is induced upon T-cell activation. NFATc2 shows 73% homology with NFATc1 within the Rel-similarity region, but has a wider tissue distribution and does not appear to be inducible. T-cell activation requires two signals for initiation, one from the antigen receptor and the other by accessory cells or agents which activate protein kinase C. Upon binding of the antigen to the T-cell receptor, several membrane events are initiated which are similar to those initiated by many hormones, including the mobilization of intracellular calcium stores.

Applications:
Suitable for use in Western Blot, Immunocytochemistry
Immunoprecipitation and Gel Supershift Assay. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: 1:250
Western Blot: 1:2000
Immunoprecipitation: Chromatin Immunoprecipitation
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D606Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Recombinant human NF-ATc1 (nuclear factor of activated T cells, cytoplasmic 1, aa1-654).
Purity:
Ascites
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human NF-ATc1 at ~105kD by Western Blot. Species Crossreactivity: mouse, rat and monkey. Does not cross-react with NF-ATc2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol. 279, C915 (2000)2. The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W.J. Leonard; EMBO J. 21, 3051 (2002) 3. Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells: A. Kerstan & T. Hunig; J. Immunol. 172, 1341 (2004) 4. Active protein kinase B regulates TCR responsiveness by modulating cytoplasmic-nuclear localization of NFAT and NF-kappa B proteins: A.K. Patra, et al.; J. Immunol. 172, 4812 (2004) 5. IL-4-Induced GATA-3 Expression Is a Time-Restricted Instruction Switch for Th2 Cell Differentiation: N. Seki, et al.; J. Immunol. 172, 6158 (2004)