Technical Data
N2566-90
Nigericin (Polyetherin A, Azalomycin M, Helixin C, Antiotic K 178, Antibiotic X 464, Pandavir)
5mg
Biochemicals Storage: -20CShipping: Blue Ice
Nigericin is a polyether antibiotic produced by Streptomyces isolated in the 1950s, notably S. hygroscopicus. Its complex structure was finally elucidated in 1968. Nigericin is an ionophore, possessing a very high affinity for monovalent cations such as Na+ and K+. Nigericin disrupts membrane potential and Golgi apparatus in mitochondria. Although nigericin can be isolated as the free acid (under acidic conditions), like most ionophores it is extracted into organic solvents and is most conveniently isolated as a salt. In vitro, nigericin has a broad biological profile activity against Gram positive bacteria, fungi, tumor cell lines and some viruses, including HIV. Nigericin is the most common member of the polyether class which are common false positives in in vitro screening bioassays using crude microbial extracts. They are thus important standards for dereplication.

Source: Streptomyces hygroscopicus

Apperance: White powder

Solubility: Soluble in DMSO, partially soluble in methanol and methanol, poor water solubility

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
28643-80-3

Molecular Formula:
C40H68O11

Molecular Weight:
724.9
Source: Streptomyces hygroscopicus
Purity: ~98%

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Nigericin, a new crystalline antibiotic from an unidentified streptomyces. Harned R.L. et al., Antibiot. Chemother. 1951, 1, 594. 2. The structure of nigericin. Steinrauf L.K. et al., Biochem. Biophys. Res. Commun. 1968, 33, 29. 3. Nigericin-induced Na+/H+ and K+/H+ exchange in synaptosomes: effect on [3H]GABA release. Rodriguez R. & Sitges M., Neurochem. Res. 1996, 21, 889. 4. Nigericin inhibits accumulation of the steroidogenic acute regulatory protein but not steroidogenesis. King S.R. et al., Mol. Cell. Endocrinol. 2000, 166, 147. 5. Nigericin inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes. Chu C.Y. et al., J. Cell. Biochem. 2002, 85, 83. 6. Inhibitory effects of polyethers on human immunodeficiency virus replication. Nakamura M., Antimicrob. Ag. Chemother. 1992, 36, 492.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.