Technical Data
Media
Nitrate Broth (Powder)
N2570
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation Per Liter: g/L
Beef Extract3.0
Peptone4.0
Potassium Nitrate1.0
Proteose Peptone No. 31.0
Total: 9.0g/L
Determination of nitrate reduction by bacteria with appropriate indicators.

Appearance:
Light to medium tan, homogeneous free flowing powder

Solubility:
Light yellow, clear, complete

pH:
As Reported


Directions per Liter: Dissolve 9 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Snell and Wright. 1941. J. Biol. Chem. 13:675. 2. Krehl, Strong and Elvehjem. 1943. Ind. & Eng. Chem., Ann. Ed. 15:471. 3. Barton-Wright. 1944. J. Biochem. 38:314. 4. United States Pharmacopeial Convention, Inc. 2001. The United States pharmacopeia, 25/The national formulary 20 2002. United States Pharmacopeial Convention, Inc., Rockville, Md. 5. Horwitz (ed.). 2000. Official methods of analysis of AOAC International, 17th ed. AOAC International, Gaithersburg, Md.