Technical Data
N2600
p-Nitrophenyl Phosphate (pNPP)
250ml
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Technical Data |
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N2600 |
p-Nitrophenyl Phosphate (pNPP) |
250ml |
| Biochemicals | Storage: 4°CShipping: Blue Ice |
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p-NPP is a single component reagent system for microwell assay of Calf Intestine Alkaline Phosphatase (CIAP or CAP) labeled probes and is an excellent substrate for phosphatase based ELISA assays. p-NPP produces a soluble end product which is yellow in color and read at 405-410 nm. Both kinetic and end point determinations can be performed. p-NPP is the customary phosphate ester used as a substrate for measuring AP activity due to its high solubility in alkaline buffers which affords zero order kinetics and significant molar extinction coefficient of 18600 mol-1cm-1. Storage and Stability: Stable for 12 months at 4°C. Also Available: N2600: p-Nitrophenyl Phosphate (pNPP) N2600-10: p-Nitrophenyl Phosphate (pNPP), 50X Concentrate, 0.19M/Liter |
Features: • Single component system • Exhibits low background levels • Safe to use - no weighing of hazardous powders • Save preparation time - use right out of the bottle • Ultra-low sensitivity • Minimal lot-to-lot variation Color: Colorless to slightly yellow Clarity: Clear Form: Supplied as a liquid in 0.95M DEA, pH 9.0 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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