Technical Data
Nitrotyrosine (nTyr) (Agarose)
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 40ul of a 50% gel slurry immunoprecipitated nitrated proteins from N2705 as detected by subsequent Western Blot analysis using 1ug/ml N2700-06.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG0.T.167Affinity Purified
100ug-20CBlue IceMouse
Purified, nitrated Keyhole Limpet Hemocyanin (KLH)
Purified by Protein G affinity chromatography.
Supplied as a liquid suspension in PBS, pH 7.4, 0.05% sodium azidewith agrose beads
Recognizes Nitrotyrosine (nTyr).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 8188 1994. 2. Ohshima, H., et al., Fund. Chem. Tox. 28: 647652, 1990. 3. Ischiropoulos, H., et al., Arch. Biochem. Biophys. 298: 431437, 1992. 4. Beckman, J.S., et al., Nature 364: 584, 1993. 5. Kooy, N.W., et al., Am. J. Respir. Crit. Care Med. 151: 12501254, 1995.