Technical Data
N2700-02A
Nitrotyrosine (nTyr)
Description:
The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in the presence of the active metabolite NO. Various pathways including the formation of peroxynitrite lead to nitrotyrosine production. It is still unknown whether nitration is merely a footprint of oxidative stress, an important pathway of nitric oxide metabolism or a part of integral processes for maintaining cellular homeostasis.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:200-1:2000
Immunoprecipitation: 1:20-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
200ul-20CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Nitrotyrosine (KLH)
Purity:
Serum
Form
Supplied as a lyophilized powder. No preservatives added. Reconstitute in 200ul sterile PBS.
Specificity:
Recognizes free and bound nitrotyrosine. Does not react with free or bound tyrosine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.