Technical Data
N2700-03A
Nitrotyrosine (nTyr) (Biotin)
Description:
The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.

Applications:
Can be used for Immunohistology on paraffin sections and frozen sections. Furthermore the antibody is useful for Western Blot and for immunoassay.

Recommended Dilution:
Immunohistology: 1:10
Western Blot: 1:10
Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b6A610Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Nitrated KLH
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.1% BSA. Conjugated to Biotin.
Specificity:
Reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. ter Steege, JCA et al; Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. Free Radic Biol Med 1998, 25: 953. 2. Beckman, JS et al; Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem 1994, 375: 81. 3. Kooy, NW et al; Evidence for in vivo peroxynitrite production in human acute lung injury. Am J Respir Crit Care Med 1995, 151: 1250. 4. Beckman, JS et al; Apperent hydroxyl radical production by peroxynitrite: Implications of endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA 1990, 87: 1620.