Technical Data
N2700-05
Nitrotyrosine (nTyr)
Description:
Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins.
Immunocytochemistry: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid.
Optimal dilutions to be determined by the researcher.

Recommended Controls:
Immunohistochemistry: Human Alzheimer diease brain sections (Methacarn fixed), Mouse livers
Western Blot: Nitrated-BSA
Indirect ELISA: Nitrated-BSA
Indirect Immunofluorescence: A549 cells metabolically labeled with 3-nitrosine

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k0.T.98Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Nitrated Keyhole Limpet Hemocyanin (KLH)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes nitrated proteins from all vertebrates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. MacMillan-Crow, L.A., et al., Proc. Natl. Acad. Sci. USA 93: 1185311858, 1996. 2. Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 8188, 1994. 3. Beckman, J.S., et al., Nature 364: 584, 1993. 4. Ischiropoulos, H., et al., Arch. Biochem, Biophys. 298: 431437, 1992. 5. Ohshima, H., et al., Ed. Chem. Tox. 28: 647652, 1990.