Technical Data
N2700-05A
Nitrotyrosine (nTyr) (HRP)
Description:
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: A 1:5000 dilution of this lot detected nitrated proteins in cells treated with increasing concentrations of peroxynitrite.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k0.T.98Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Nitrated KLH
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris, pH 7.4, 0.15M sodium chloride, 0.01% merthiolate prior to the addition of glycerol to 30%. Conjugated to HRP.
Specificity:
Recognizes nitrated proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 81-88, 1994.
2. Ohshima, H., et al., Ed. Chem. Tox. 28: 647-652, 1990.
3. Ischiropoulos, H., et al., Arch. Biochem, Biophys. 298: 431-437, 1992.
4. Beckman, J.S., et al., Nature 364: 584, 1993.