		Technical Data
N2700-05A	Nitrotyrosine (nTyr) (HRP)

Description:
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: A 1:5000 dilution of this lot detected nitrated proteins in cells treated with increasing concentrations of peroxynitrite.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	0.T.98	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice		Mouse

Concentration:

Not determined

Immunogen:

Nitrated KLH

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris, pH 7.4, 0.15M sodium chloride, 0.01% merthiolate prior to the addition of glycerol to 30%. Conjugated to HRP.

Specificity:

Recognizes nitrated proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 81-88, 1994.
2. Ohshima, H., et al., Ed. Chem. Tox. 28: 647-652, 1990.
3. Ischiropoulos, H., et al., Arch. Biochem, Biophys. 298: 431-437, 1992.
4. Beckman, J.S., et al., Nature 364: 584, 1993.