Technical Data
Nitrotyrosine (nTyr)
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Suitable for use in detection of nitrated tyrosine, ELISA and Immunoblot. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:20,000-1:160,000
Western Blot: 1:20,000-1:160,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

1ml4C (-20C Glycerol)Blue IceGoat
Not determined
Purified, nitrated Keyhole Limpet Hemocyanin (KLH)
Neat serum
Supplied as liquid with 0.09% sodium azide.
Specifically binds to nitro-LDL and other nitrated proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Franze, T. et al., Analyst (2004) 129:589-596. 1. Beckman, et al., Biological Ehemistry Hoppe-Seyler, 375: 81 (1994). 2. Haddad, et al., J. Clin. Invest., 94: 2407 (1994).