Technical Data
Nitrotyrosine (nTyr)
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:200-1:40,000
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
1mg-20CBlue IceGoat
Pure nitrated Keyhole Limpet Hemocyanin (NY-KLH)
Purified by Nitrated protein-sepharose affinity chromatography.
Supplied as a liquid in PBS, 1mM EDTA, 0.02% sodium azide.
Specifically binds to nitrate LDL and other nitrated proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.