		Technical Data
N2700-12A	Nitrotyrosine (nTyr)

Description:
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:200-1:40,000
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice		Goat

Concentration:

~1mg/ml

Immunogen:

Pure nitrated Keyhole Limpet Hemocyanin (NY-KLH)

Purity:

Purified by Nitrated protein-sepharose affinity chromatography.

Form

Supplied as a liquid in PBS, 1mM EDTA, 0.02% sodium azide.

Specificity:

Specifically binds to nitrate LDL and other nitrated proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.