Technical Data
N2700-13D
Nitrotyrosine (nTyr)
Description:
The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT (MTT detection) or 0.1ug/ml for HRPO/ECL detection.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6D609Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
KLH modified with nitrotyrosine
Purity:
Purified by thiophilic adsorption and size exclusion chromatography.
Form
Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute in 1ml sterile ddH2O.
Specificity:
Recognizes Nitrotyrosine (nTyr). Species Crossreactivity: human, mouse, rat and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.