Technical Data
N2700-16B
Nitrotyrosine, Conjugated
Description:
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunocytochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Compound Cross-reactivity ratio (a)
NO2-Tyrosine-G-BSA 1
NO-Tyr-G-BSA 1/250
Tyr-G-BSA 1/> 100,000
NO2-5HT-G-BSA 1/> 100,000
NO-5HT-G-BSA 1/> 100,000
NO-Cys-G-BSA 1/> 100,000
NO-Trp-G-BSA 1/> 100,000
NO-His-G-BSA 1/> 100,000

NO2-Tyrosine-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement.
G = Glutaraldehyde, BSA= Bovine Serum Albumin
Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to NO2-Tyrosine (BSA).
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2. 40% glycerol. No preservative added.
Specificity:
Recognizes protein-linked NO2-L-Tyrosine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.