Technical Data
Nitrotyrosine (nTyr)
Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis.

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
ELISA: 2-100ng/ml
Western Blot: 0.1ug/ml
Immunoprecipitation: 1ug/ml
Optimal dilutions to be determined by the researcher.

Crossreactivity Data:
Specificity as determined by ELISA competition experiments.

Compound Inhibition
Nitrotyrosine 95%
Nitrotryptophan 4%
Chlorotyrosine 3%
Phosphotyrosine 2%
Tyrosine 0%
Aminotyrosine 0%

Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo
I1904-65N: IgG (HRP) Pab Rb xMo
I1903-07E: IgG (HRP) Pab Rb xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG110A9Affinity Purified
100ug-20CBlue IceMouse
Protein-bound Nitrotyrosine
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro hemoglobin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.