Technical Data
N2700-16D
Nitrotyrosine (nTyr)
Description:
Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
ELISA: 2-100ng/ml
Western Blot: 0.1ug/ml
Immunoprecipitation: 1ug/ml
Optimal dilutions to be determined by the researcher.

Crossreactivity Data:
Specificity as determined by ELISA competition experiments.

Compound Inhibition
Nitrotyrosine 95%
Nitrotryptophan 4%
Chlorotyrosine 3%
Phosphotyrosine 2%
Tyrosine 0%
Aminotyrosine 0%

Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo
I1904-65N: IgG (HRP) Pab Rb xMo
I1903-07E: IgG (HRP) Pab Rb xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110A9Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Protein-bound Nitrotyrosine
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro hemoglobin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.