Technical Data
N2750-10
NIS (Sodium Iodide Symporter)
Description:
Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane. Suitable for studying the sensitivity of thyroid tumors to iodine treatment..

Applications:
Suitable for ELISA, Immunohistochemistry and Immunofluorescence Microscopy. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen sections): 1:1200-1:2000 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 4C.
Optimal dilution to be determined by researcher.

Positive Control:
Frozen sections of rat thyroid.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide from the C-terminus of rat NIS.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 50-100ul of sterile ddH2O. Dilute further with 0.1M PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognzies rat NIS. Species Crossreactivity: porcine and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.