		Technical Data
N2750-10	NIS (Sodium Iodide Symporter)

Description:
Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane. Suitable for studying the sensitivity of thyroid tumors to iodine treatment..

Applications:
Suitable for ELISA, Immunohistochemistry and Immunofluorescence Microscopy. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen sections): 1:1200-1:2000 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 4°C.
Optimal dilution to be determined by researcher.

Positive Control:
Frozen sections of rat thyroid.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

Not Determined

Immunogen:

Synthetic peptide from the C-terminus of rat NIS.

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 50-100ul of sterile ddH2O. Dilute further with 0.1M PBS, 1% BSA, 0.1% sodium azide.

Specificity:

Recognzies rat NIS. Species Crossreactivity: porcine and human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.