Technical Data
N2904-15
NMDA 1 Receptor, CT, C2 Cassette (NMDA R1, N-methyl-D-aspartate)
Description:
The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy- 5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100-1:500
Immunohistochemistry: 1:100-1:500. Using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b2Q155Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to LQNQKDTVLPRRAIEREEGQLQLCSRHRES from the C-terminus of rat NMDA receptor subunit.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes rat NMDAR1, C-terminal, C2 cassette.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.