Technical Data
N2940-10
Nitroso-L-Histidine (NO-Histidine), Conjugated
Description:
Applications: Suitable for use in ELISA. Other applications not tested.

Specificity
Using a conjugate NO-Histidine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds.

Compound Cross-reactivity ratio (a)
NO-Histidine-G-BSA 1
Histidine-G-BSA 1/>100 000
NO-Cysteine-G-BSA 1/>100 000
NO-5HT-G-BSA 1/>100 000
NO-Tryptophan-G-BSA 1/65
NO-Tyrosine-G-BSA 1/307


(a) : NO-Histidine-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin

Recommended dilution
The anti-conjugated NO-Histidine antibodies can be diluted between 1/2,000-1/5,000.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRat
Concentration:
~1mg/ml
Immunogen:
NO-Histidine-Glutaraldehyde-BSA
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein-conjugated NO-L-Histidine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.