Technical Data
N2940-90
Nitroserotonin (NO2-Serotonin), Conjugated
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Specificity:
Using a conjugate NO2-Serotonin-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compound Cross-reactivity ratio
NO2-5HT-G-BSA 1
5HT-G-BSA 1:100,000
NO2-5HT 1:100,000
NO-5HT-G-BSA 1525
NO-Cys-G-BSA 1:100,000
NO-W-G-BSA 1:100,000
NO-His-G-BSA 1:100,000

NO2-Serotonin-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement; G = Glutaraldehyde

Recommended Dilution:
The anti-conjugated NO2-Serotonine antibodies can be diluted between 1/2,000-1/5,000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceRabbit
Concentration:
~1mg/ml
Immunogen:
NO2-Serotonin-Glutaraldehyde-BSA
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein-conjugated NO2-Serotonin. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.