Technical Data
N2950-06B
nNOS (Neuronal Nitric Oxide Synthase)
Description:
Nitric oxide (NO) is a potent bio-regulatory molecule which plays important roles in the regulation of a variety of normal developmental and physiological processes. NO is generated by nitric oxide synthase (NOS) in a reaction which converts L-arginine and oxygen into citrulline and nitric oxide. Three distinct mammalian genes have been identified which encode NOS isoforms: neuronal (nNOS or bNOS), macrophage or inducible (iNOS) and endothelial (eNOS). The NOS isoforms can be subdivided into two general categories, constitutive or inducible, based on differences in their regulation and activities. The constitutive isoforms include eNOS and nNOS. Although the nomenclature of the NOS family members suggests restricted isoform expression patterns, the160kD neuronal-type NO synthase (nNOS) has been detected in a variety of cell types including neurons, epithelial cells, mesangial cells and skeletal muscle cells.

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot (Rat): 1ug/ml
ELISA: 0.1-1ug/ml
Immunoprecipitation (Rat): 7ug/each
Immunohistochemistry (Formalin- fixed paraffin): 2ug/ml. Tests using human, mouse and rat brain tissue sections yielded positive and specific cytoplasmic staining in neurons and astrocytes.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6A143Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein derived from the N-terminal region of rat nNOS.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Specificity:
Recognzies rat nNOS at ~160kD. Reactivity has been confirmed with rat brain tissue lystaes. Species Crossreactivity: Mouse, human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.