Technical Data
Nociceptin (Orphanin FQ)
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:500
This orphanin FQ robustly stains nerve fibers in brain and spinal cord. Animals were treated with colchicines to visualize orphanin FQ-expressing cell bodies. Staining was blocked by preabsorption with the cognate peptide.

Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.

Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul-20CBlue IceRatRabbit
Not determined
Synthetic peptide corresponding to residues 122-138 of rat Orphanin FQ.
Neat serum.
Supplied as a liquid with 0.05% sodium azide.
Species Reactivity: Rat and Primate.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.